EMP2 overexpression induces cell death on growth factor withdrawal.

(A) DAC or MV cells (3 × 10⁵) were cultured in 0.5% FCS for 2 days, and viable cells were enumerated by trypan blue exclusion. MV cells had a 30% higher survival rate than DAC cells. (B) NIH3T3 cells (3 × 10⁵) overexpressing EMP2 at various levels, or infected with empty vector, were plated overnight in 10% FCS, washed, and then incubated for 2 days in medium containing 0.5% FCS. Cells were harvested, and viable cells were enumerated using trypan blue exclusion. EMP2 expression inversely correlated with survival in fibroblasts.