Fig. 1.
Agarose gel electrophoresis of positive samples.
The nested PCR was performed as described in “Study design,” and the PCR products were loaded on a 2% agarose electrophoresis gel containing ethidium bromide and visualized under UV light. (A) Japanese samples showing a single band (lanes 1-33). (B) Japanese samples showing multiple bands (lanes 1-6). (C) German samples showing a single band (lanes 1-22). (D) German samples showing multiple bands (lanes 1-17). Lane M shows 100-bp ladder markers.