Enforced expression of FucT-VII in a C2GlcNAcT-I⁻ lymphoid cell line confers binding to E-selectin.

(A) Expression of FucT-VII and C2GlcNAcT-I in 300.19 cells and 300.19/FucT-VII cells. RT-PCR analysis was performed as described in “Materials and methods.” Murine bone marrow and U937 cells were used as positive controls. Levels of FucT-VII mRNA in 300.19/FucT-VII/C2GlcNAcT-1(L) cells were similar to those of FucT-VII in 300.19/FucT-VII cells (data not shown). (B) Rolling of 300.19, 300.19/FucT-VII, and 300.19/FucT-VII/C2GlcNAcT-I cells on CHO/E-selectin. Data are presented as mean ± SD of total rolling events. (C) Rolling velocity of 300.19, 300.19/FucT-VII, and 300.19/FucT-VII/C2GlcNAcT-I cells on E-selectin. Rolling velocity (± SD) of cells from panel B are presented. For both panels B and C, U937 cells are included for comparison and as a positive control. Data represent one of 4 similar experiments. Asterisk indicates statistically different (P < .05) from 300.19/FucT-VII.