Fig. 3.
![Fig. 3. Nonlinear relationship of fraction of sickle Hb and percentage of sickle RBCs. / A graph of the percent HbS as measured by HPLC versus a visual estimate of the percent sickle cell mouse–derived RBCs is compared to a predicted curve based on the difference in MCH between transgenic sickle cell mice (MHC = 9.3 pg/cell) and normal donor mice (MHC = 14.5 pg/cell). The predicted curve is based on the following formula: % Hemoglobin S = {[(% sickle RBCs)(9.3 pg/cell)] × 100}/{[(% sickle RBCs)(9.3 pg/cell)] + [(% normal RBCs)(14.5 pg/cell)]}.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/12/10.1182_blood.v97.12.3960/6/h81211164003.jpeg?Expires=1735813281&Signature=0bajBxAM1zt2j2P6Fv~EWtrPKTJnQ7mX9dFjQLRPFjNf2j-gfoo1DqPEVmmM-3Y-gxpuODJ2EvuACZLdL0iwyYiwW9XyeJ7iHT5whE-p4~14voHHdW8zQirbVxeR-pK~6JfIgfMQiZv79gA1eDdnkqKVCbApFVkcuTimcANUc7BCh5dku76kJje6cVcZdyJUoAsQ7b8EvrBSOxpyT6Ar2VgPBFauKBlVo0HA4ZWlRD6d-Qu6mPyBYZPBuJ5gf5czpJaMtI8Vtiwx5kkkqCRL4BHZFsKtJ~ZHRLva9ubDkFolJ87L~a1-2ic53jxAHt1b~VI1xRdXBORmiuxOxw5NFw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Nonlinear relationship of fraction of sickle Hb and percentage of sickle RBCs.
A graph of the percent HbS as measured by HPLC versus a visual estimate of the percent sickle cell mouse–derived RBCs is compared to a predicted curve based on the difference in MCH between transgenic sickle cell mice (MHC = 9.3 pg/cell) and normal donor mice (MHC = 14.5 pg/cell). The predicted curve is based on the following formula: % Hemoglobin S = {[(% sickle RBCs)(9.3 pg/cell)] × 100}/{[(% sickle RBCs)(9.3 pg/cell)] + [(% normal RBCs)(14.5 pg/cell)]}.
