American Society of Hematology

Fig. 1.

$Fig. 1. Immunophenotype of diagnostic bone marrow and flow cytometric analysis of and detection of TCR rearrangement in the progenitor population of the remission marrow in patient 7. / (A) Expression of CD34, CD38, and CD19 in the diagnostic bone marrow. (B) The day 28 remission marrow was enriched for CD34+cells and analyzed for expression of CD34 and CD38. Quadrants containing cells expressing CD34+CD38+ and CD34+CD38− were designated as the sorting regions R2 and R3, respectively. (C) Cells fractionated into CD34+CD38+ and CD34+CD38− underwent PCR amplification and Southern hybridization, along with other controls as appropriate, for detection of the clonotypic Vδ2-Dδ3 rearrangement originally identified in this patient. The B-actin gene was used as a control for the presence of DNA in the fractions tested. MNC indicates mononuclear cells.$

Immunophenotype of diagnostic bone marrow and flow cytometric analysis of and detection of TCR rearrangement in the progenitor population of the remission marrow in patient 7.

(A) Expression of CD34, CD38, and CD19 in the diagnostic bone marrow. (B) The day 28 remission marrow was enriched for CD34⁺cells and analyzed for expression of CD34 and CD38. Quadrants containing cells expressing CD34⁺CD38⁺ and CD34⁺CD38⁻ were designated as the sorting regions R2 and R3, respectively. (C) Cells fractionated into CD34⁺CD38⁺ and CD34⁺CD38⁻ underwent PCR amplification and Southern hybridization, along with other controls as appropriate, for detection of the clonotypic Vδ2-Dδ3 rearrangement originally identified in this patient. The B-actin gene was used as a control for the presence of DNA in the fractions tested. MNC indicates mononuclear cells.

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