TFPI-1 on mononuclear cells is GPI-anchored.

Effect of phospholipase C treatment on the association of TFPI-1 with mononuclear cells. Cells were incubated in the absence and in the presence of phospholipase C. TFPI-1 surface expression was analyzed by flow cytometry (A) and TX-114-soluble, membrane-associated TFPI-1 by Western blotting with anti–FPI-1 antibody (B).