Nicotine inhibits hematopoiesis in LTBMCs.
LTBMCs were treated weekly without (○) or with 10−5 M nicotine added from the first week of culture (●) and the fifth week of culture (▪). (A) Nonadherent cells were harvested weekly during feedings of the cultures (n = 4) and calculated, and numbers were expressed as mean ± SD. (B) Nonadherent cells harvested from LTBMCs treated with nicotine were plated at a concentration of 104 cells/mL in methylcellulose cultures (n = 4) supplemented with GM-CSF (10 ng/mL). After 7 days in culture, the number of colonies was counted, recalculated for a total number of cells for each culture, and expressed as mean ± SD. (C) LTBMCs were cultured with nicotine or cotinine for 3 weeks. Thereafter, the adherent layer of LTBMCs was harvested, calculated, and tested for LTC-IC content in a 14-day limiting-dilution assay on the S17 cell line. Data shown are means ± SD of the LTC-IC content of 2 experiments.
