Expression of RCAS1R on bone marrow cells from a healthy volunteer.

The expression of RCAS1R/glycophorin A/TfR was examined on bone marrow cells from normal volunteers. The analysis was performed after incubation for 16 hours in IMDM containing 15% FBS, 15% human AB serum, rhEPO (2 U/mL), rhSCF (20 ng/mL), rhIL-3 (100 ng/mL), and rhGM-CSF (50 ng/mL). Flow cytometry analysis was performed after 2-color staining of RCAS1R versus glycophorin A, or RCAS1R versus TfR. Region A was first gated by selecting the population that contained abundant erythroid progenitor cells in a FSC versus SSC dot plot (A). Then, expressions of glycophorin A versus RCAS1R (B) and TfR (CD71) versus RCAS1R (C) were assessed in region A cells. The results shown are for one experiment representative of the 4 performed.