Effect of VEGF on MM and PCL cell migration.

VEGF activates cell migration in MM and PCL cells. Growth-factor–deprived MM.1S (panel A), patient MM (panel B), and patient PCL (panel C) cells were plated on a polycarbonate membrane (8-μm pore size) in a modified Boyden chamber and exposed for 4 hours to VEGF (0.5 to 500 ng/mL) added to the lower chamber. At the end of the treatments, cells on the lower part of the membrane were counted with a Coulter counter ZBII. The results shown for MM.1S cells are representative of 3 independent experiments.