PKC dependency of VEGF-induced migration.

Growth-factor–deprived MM.1S cells (panel A) were pretreated with or without BIM or PD89059, plated on a polycarbonate membrane (8-μm pore size) in a modified Boyden chamber, and exposed for 4 hours to VEGF (0.5 and 5 ng/mL) added to the lower chamber. At the end of the treatments, cells on the lower part of the membrane were counted with a Coulter counter ZBII. Growth-factor–deprived patient PCL cells (panel B) were pretreated with BIM, and migration induced by VEGF (5 or 50 ng/mL) was analyzed as described above. Results are expressed as the fold migration of untreated cells (control). Representative results from 2 separate experiments are shown.