Change of active oxygen-generating ability in patients' neutrophils and surface cytochrome

b₅₅₈ expression after IFN-γ treatment. Active oxygen production in neutrophils obtained from the patients at the indicated days after IFN-γ treatment was analyzed by flow cytometry using DHR-123 as a fluorescence probe and PMA as a stimulus. (A) Increase in fluorescence intensity in comparison to control cells was estimated and expressed as percent increase in mean fluorescence. (B) Surface expression of cytochromeb₅₅₈ in neutrophils at the indicated days were analyzed by flow cytometry after staining with anticytochromeb₅₅₈ monoclonal antibody 7D5. The lines indicate the mean values.