Splicing pattern change of

CYBB gene transcripts after IFN-γ treatment.From neutrophil RNA of the patients who received IFN-γ 26 days before, single-stranded cDNAs were synthesized. PCR products were obtained from the cDNAs by using 2 sets of primers, a forward primer on exon 1 and a reverse primer on exon 4 (A) or a forward primer on exon 1 and a reverse primer on exon 6 (B,C). The products were separated on a 3% agarose gel and stained with ethidium bromide. In panels A and B, 40-cycle and in panel C, 50-cycle amplifications were performed. DNA size markers are shown on left and numbers shown on right indicate differently spliced products illustrated in Figure 3.