Anti-CD154 mAb administration results in donor chimerism of allogeneic bone marrow in more than 90% of recipients receiving a nonmyeloablative conditioning regime of 200 cGy TBI.
B6 mice were irradiated on day −1 and infused with 40 × 106 BALB/c bone marrow cells on day 0. Irrelevant hIgG or anti-CD154 mAb was administered from day 1 through day 14 after BMT. PBLs were typed for percentage donor-host chimerism at 6 weeks after BMT. Ten mice per group underwent transplantation. The graph represents a pool of 10 experiments. No hIgG-treated mice had any level of detectable donor chimerism (not shown). On the x-axis are ranges of percentage donor engraftment. On the y-axis are percentages of mice in each donor percentile. The total number of mice in each percentage donor range is shown above the column. The average percentage donor engraftment for all 99 mice was 48% ± 19%.
