Anti-CD154–facilitated alloengraftment is multilineage.

Twenty mice from 2 representative experiments shown in Figure 1 were phenotyped at 120 days after BMT for donor-host origin of CD4⁺ and CD8⁺ T cells, CD19⁺ B cells, and MAC-1⁺ myeloid cells. On the x-axis are shown the host and donor proportions of each of the lineages. ▪ indicates the proportion of each lineage of host origin; ▥, the proportion of each lineage that is of donor origin. On the y-axis is shown the percentage of PBLs of each lineage. Irrelevant hIgG–treated mice had no detectable donor chimerism and thus are composed entirely of host-type cells. Note that most CD4⁺ T cells, CD19⁺ B cells, and MAC-1⁺ myeloid cells in anti-CD154–treated mice are of donor origin. In contrast, most of the CD8⁺ T cells are of host origin.