Fig. 3.
IL-12–induced STAT4 DNA-binding activity in activated T cells cultured with cytokines.
Activated T cells were cultured with or without cytokines, as indicated, for 24 hours. All cells were then treated with IL-12 for 20 minutes. Nuclear extracts were prepared, and an EMSA was performed in the absence or presence of antibody to STAT4 as indicated. S: STAT4 and DNA complex. SS: Complex formed by STAT4, DNA, and anti-STAT4 antibody.
