Fig. 7.
Differential effects of MG-132 on STAT4 protein in IL-12 and INF-α signaling pathways.
Activated T cells were cultured with the proteasome inhibitor MG-132 (10 nM) or DMSO for 1 hour and then cultured with or without cytokines as indicated for 6 hours. Whole-cell extracts were prepared, and Western blots were probed with anti-STAT4 (top panel). The membrane was reprobed with anti-STAT1 as a quantity control (bottom panel).
