Similar percentages of Lyn-deficient mast cells express c-Kit as wild-type mast cells.

Pooled mononuclear cells from the bone marrow (n = 2) of wild-type or Lyn-deficient mice were cultured in murine IL-3 for 5 weeks. FACS analysis was performed to compare the expression of c-Kit and high-affinity IgE receptors, 2 mast-cell markers, on the cell surface. Nonshaded peaks represent cells stained with isotype-matched control antibody. Shaded peaks represent cells stained with either IgE or c-Kit antibodies, as indicated. MFI represents the average level of high-affinity IgE receptor or c-Kit expression on the cell surface. Data are representative of the results of triplicate experiments. FITC indicates fluorescein isothiocyanate.