SCF-induced migration of Lyn-deficient mast cells is reduced.

(A) Impaired migration of Lyn-deficient mast cells is dose-related. Wild-type (♦) or Lyn-deficient (▪) mast cells were assessed for migration in response to the indicated concentration of murine SCF using a modified Boyden chamber as described in “Materials and methods.” Data represent the mean and standard deviation of triplicate samples and are representative of the results of 4 independent experiments. (B) PMA-induced migration of BMMCs from Lyn-deficient mice is normal. Migration of BMMCs from wild-type (■) or Lyn-deficient (░) mice in response to murine SCF (100 ng/mL) or PMA (50 ng/mL). All samples were performed in triplicate, and results are representative of 2 independent experiments. (C) SCF-induced migration of BMMCs is not the result of chemokinesis. Migration of BMMCs from wild-type (■) or Lyn-deficient (░) mice was performed as described above, except that the percentage of migrating cells was assessed in the presence of media or SCF in the top or bottom wells of the chemotaxis chamber, as indicated.