American Society of Hematology

Fig. 3.

$Fig. 3. Cytochrome c-mediated activation of caspases in cell-free extracts. / (A) Western blot analysis of cytochrome c in cytosolic fractions from undifferentiated (U937) and TPA-differentiated (U-TPA) U937 cells treated (+) or not treated (−) with 50 μM etoposide for 4.5 hours. (B) Cell-free extracts from U937 and U-TPA cells were left untreated for 2 hours (TE) or were incubated for indicated times (minutes) in the presence of 5 μM cytochrome c and 1 mM dATP. Processing of indicated caspases was analyzed by Western blot. Numbers are molecular weights in kilodaltons. *Cleavage products. Loading was checked with the use of an antihuman HSC70 mAb.$

Cytochrome c-mediated activation of caspases in cell-free extracts.

(A) Western blot analysis of cytochrome c in cytosolic fractions from undifferentiated (U937) and TPA-differentiated (U-TPA) U937 cells treated (+) or not treated (−) with 50 μM etoposide for 4.5 hours. (B) Cell-free extracts from U937 and U-TPA cells were left untreated for 2 hours (TE) or were incubated for indicated times (minutes) in the presence of 5 μM cytochrome c and 1 mM dATP. Processing of indicated caspases was analyzed by Western blot. Numbers are molecular weights in kilodaltons. *Cleavage products. Loading was checked with the use of an antihuman HSC70 mAb.

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