American Society of Hematology

Fig. 4.

$Fig. 4. TPA-induced differentiation of U937 cells does not alter mitochondrial reactivity. / (A) Flow cytometry analysis of mitochondrial potential in undifferentiated (U937) and TPA-differentiated (U-TPA) U937 cells, treated (+) or not treated (−) with 100 μM m-chlorophenylhydrazone for 30 minutes. Mitochondrial membrane depolarization is indicated by the decreased mitochondrial aggregate (FL2-H) and the increased cytosolic monomer (FL1-H). Dashed lines indicate the controls. (B) Purified mitochondria isolated from U937 and U-TPA cells were treated (+) or not treated (−) with 5 mM atractyloside for 30 minutes. Cytochrome c expression was analyzed by Western blot in mitochondrial (Mit) and supernatant (Buffer) fractions. Lack of supernatant contamination by mitochondria extracts was checked by using an antihuman cytochrome oxidase subunit IV (COX IV) mAb.$

TPA-induced differentiation of U937 cells does not alter mitochondrial reactivity.

(A) Flow cytometry analysis of mitochondrial potential in undifferentiated (U937) and TPA-differentiated (U-TPA) U937 cells, treated (+) or not treated (−) with 100 μM m-chlorophenylhydrazone for 30 minutes. Mitochondrial membrane depolarization is indicated by the decreased mitochondrial aggregate (FL2-H) and the increased cytosolic monomer (FL1-H). Dashed lines indicate the controls. (B) Purified mitochondria isolated from U937 and U-TPA cells were treated (+) or not treated (−) with 5 mM atractyloside for 30 minutes. Cytochrome c expression was analyzed by Western blot in mitochondrial (Mit) and supernatant (Buffer) fractions. Lack of supernatant contamination by mitochondria extracts was checked by using an antihuman cytochrome oxidase subunit IV (COX IV) mAb.

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