Fig. 3.
The effect of the pgp reversal agent PSC833 on the MFI of DiOC6 after treatment with a range of mClCCP doses.
PSC833 (2 μM) was added to cultures immediately before mClCCP. Although the total MFI of PSC-833–treated cells (open diamonds) was increased compared with that in untreated cells (solid diamonds; plot A), treated cell fluorescence as a percentage of control fluorescence was the same in treated and untreated samples (plot B). These results are representative of 3 similar experiments on AML samples selected as having high pgp expression from a bank of more than 200 assayed samples. The pgp was measured by using the MRK-16 antibody and the rhodamine modulation assay as described previously.45
