Loss of cell viability in primary AML blasts after overnight culture.

Apoptotic cell death was determined by flow cytometry using 7-AAD. (A) Dose-response curve for mClCCP. The data plotted represent the mean (SD) value from 5 different cases analyzed in separate experiments. (B) Dot plots for an individual case. Two gated regions are shown. R3 encompasses cells with high forward scatter that exclude 7-AAD (viable cells). Apoptotic cells, which take up 7-AAD and have a reduced forward scatter, indicative of shrinking, are shown in R4. (i) Control cells (21% in R4). (ii) Cells cultured overnight with 30 μM mClCCP (59% in R4). (C) The mClCCP IC₅₀ for 12 AML cases plotted against the percentage of cells that did not survive 21 to 23 hours of suspension culture without added growth factors.