Cofactor dependency of the purified CIC monoclonal IgG Abs and of the CIC serum.

Monoclonal Abs were tested for their reactivities with anionic PL, CL (A) and PS (B) in the presence of purified human proteins. The results are expressed as the percentage of increase of OD in the presence of the purified cofactors. The patient's serum was tested diluted at 1:100, and the purified IgG was tested at a concentration of 5 mg/mL. Lane a: BSA (control); lane b: β2GP1; lane c: prothrombin; lane d: Prot S; lane e: Prot C; lane f: An V.