Time and dose dependency of aggregation induced by CD98-J3/AHN-18.

(A) U937 cells were incubated with CD98-AHN-18 (1 μg/mL), MEM 101A (CD29; 0.3 μg/mL), or 161-46 (CD43, 0.3 μg/mL) for various times as shown. Aggregation was measured as described in “Materials and methods.” The results show mean aggregation from triplicate cultures for 1 representative experiment of 2. (B) U937 cells were incubated with CD98-AHN-18 or fragments of CD98-AHN-18 at different concentrations (μg/mL) for 6 hours. Aggregation and binding were measured as for Figure 1. The results show mean aggregation ± SEM for triplicate cultures for 1 representative experiment of 2.