CD98-AHN-18 antibody does not induce apoptosis of U937 cells or inhibit cell division.
(A) U937 cells, 5 × 105 were incubated in the presence of different concentrations of CD98-AHN-18 antibody or isotype control in complete medium. MTT reduction was measured after 48 hours. Results are expressed as percentage of OD (mean ± SEM for triplicate cultures), relative to OD in the absence of antibody. The isotype control significantly stimulated MTT reduction at the highest concentration (P < .01), but all other values did not differ significantly from control. (B) U937 cells, 5 × 106 were seeded into 96 well-plates, and incubated for 24 or 48 hours in the presence of CD98-AHN-18 antibody (1.5 μg/mL) or an isotype control. Cells were harvested and counted by means of trypan blue staining and light microscopy. The results show mean ± SEM for triplicate cultures. No value differed significantly from control.
