Flow cytometry analysis of CD36 and α₄β₁ on RBCs and reticulocytes from SS patients.

Typical profiles showing the simultaneous staining with anti-CD36 (panels A, B) or anti-α₄β₁ (panels C, D) antibody and thiazole orange of erythroid cell populations (RBCs and reticulocytes) from CD36⁻ (panels A, C) and CD36⁺ (panels B, D) patients homozygous for Hb S (SS). Mature RBCs, mature reticulocytes, and immature reticulocytes were gated in regions 1, 2, and 3, respectively, according to the intensity of staining with the reagents used. Inserts in the upper left corners show histograms illustrating the heterogeneous expression of CD36 (panel B) and α₄β₁ (panels C, D) on reticulocytes gated in both regions 2 and 3.