Iron stain in spleen and liver in wild-type and heterozygous

Fth^+/− mice. Perls Prussian blue staining of liver (panel A) or spleen (panels B-F) from wild-type (panels A-C) or heterozygous (panels D-F) animals. Animals were analyzed at 6 weeks (panels B,D), 10 weeks (panel E), or 25 weeks (panels A,C,F) after birth. In both genotypes, there is a progressive iron loading of the spleen red pulp, with very few blue deposits in the white pulp, whereas there is no stainable iron in the liver (× 100).