Binding of 12E4 phages, 12G1 phages, and 27A10 phages to mAbs 12E4, 12G1, or 27A10.

Phages bound specifically to the mAb on which they were selected. One of the phages, selected on mAb 12G1 (phage 1 (■), cross-reacted with mAb 12E4. Binding of the phages was detected using HRP-labeled anti-M13 antibody, as described in “Materials and methods.” ▪, 12E4 phage and 12E4 mAb; ▾, 12G1 phage 1 and 12G1 mAb; ▿, 12E4 phage and 12G1 mAb; ▵, 27A10 phage and 12G1 mAb; ♦, 27A10 phage and 27A10 mAb; ⋄, 12G1 phage 1 and 27A10 mAb.