Fig. 1.
Fig. 1. MTT assay results. / Assays on AML blasts were carried out in 96-well plates. Drugs were prepared at 2 × concentration in complete medium and 50-μL aliquots were added to wells. Control wells contained DMSO in medium (final 0.125%) for AG1295 experiments, or phosphate-buffered saline (PBS) alone for Ara C experiments. Blasts were resuspended at 4 × 106/mL and 50-μL aliquots were added to the wells (final 200 000 cells/well in 100 μL volume). After 72 hours of incubation at 37°C in 5% CO2, the plates were assayed for MTT uptake. Optical densities (OD) were determined by spectrophotometry at 562 nm. The y-axis of each graph displays the mean OD of the drug treatments expressed as a percentage of the untreated (DMSO or PBS) controls. (A) Response of AML samples to 5 μM AG1295. Blasts were incubated as described with 5 μM AG1295 for 72 hours in duplicate or triplicate wells of 96-well plates before subjecting them to the MTT assay. All 23 samples (8 FLT3/ITD and 15 non-ITD) are shown in this scatterplot, grouped according to their FLT3/ITD status. TheP value shown refers to the difference between these 2 groups and is based on a 2 sample t test. (B) Dose-response curve of FLT3/ITD and non-ITD AML samples treated with AG1295. Each of the 23 samples shown in panel A was incubated with increasing concentrations of AG1295 for 72 hours prior to the MTT assay. The data are grouped according to FLT3/ITD status and a mean response for each AG1295 concentration was calculated. Errors bars represent the SD of the mean OD of the treatment points expressed as a percentage of the mean OD of the control wells. Each point on the FLT3/ITD curve represents the mean of 8 samples; those on the non-ITD curve represent that of 15 samples. (C) Dose-response curves for AG1295 versus Ara C. A FLT3/ITD AML sample and a non-ITD sample were each exposed to increasing concentrations of either AG1295 or Ara C for 72 hours prior to the MTT assay. Error bars represent the SD of the OD of the triplicate samples at each concentration, expressed as a percentage of the mean OD of the control wells. The x-axis displays the micromolar concentration of either AG1295 or Ara C.

MTT assay results.

Assays on AML blasts were carried out in 96-well plates. Drugs were prepared at 2 × concentration in complete medium and 50-μL aliquots were added to wells. Control wells contained DMSO in medium (final 0.125%) for AG1295 experiments, or phosphate-buffered saline (PBS) alone for Ara C experiments. Blasts were resuspended at 4 × 106/mL and 50-μL aliquots were added to the wells (final 200 000 cells/well in 100 μL volume). After 72 hours of incubation at 37°C in 5% CO2, the plates were assayed for MTT uptake. Optical densities (OD) were determined by spectrophotometry at 562 nm. The y-axis of each graph displays the mean OD of the drug treatments expressed as a percentage of the untreated (DMSO or PBS) controls. (A) Response of AML samples to 5 μM AG1295. Blasts were incubated as described with 5 μM AG1295 for 72 hours in duplicate or triplicate wells of 96-well plates before subjecting them to the MTT assay. All 23 samples (8 FLT3/ITD and 15 non-ITD) are shown in this scatterplot, grouped according to their FLT3/ITD status. TheP value shown refers to the difference between these 2 groups and is based on a 2 sample t test. (B) Dose-response curve of FLT3/ITD and non-ITD AML samples treated with AG1295. Each of the 23 samples shown in panel A was incubated with increasing concentrations of AG1295 for 72 hours prior to the MTT assay. The data are grouped according to FLT3/ITD status and a mean response for each AG1295 concentration was calculated. Errors bars represent the SD of the mean OD of the treatment points expressed as a percentage of the mean OD of the control wells. Each point on the FLT3/ITD curve represents the mean of 8 samples; those on the non-ITD curve represent that of 15 samples. (C) Dose-response curves for AG1295 versus Ara C. A FLT3/ITD AML sample and a non-ITD sample were each exposed to increasing concentrations of either AG1295 or Ara C for 72 hours prior to the MTT assay. Error bars represent the SD of the OD of the triplicate samples at each concentration, expressed as a percentage of the mean OD of the control wells. The x-axis displays the micromolar concentration of either AG1295 or Ara C.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal