American Society of Hematology

Fig. 2.

$Fig. 2. Apoptosis assay. / Blasts from a FLT3/ITD sample and a non-ITD sample were incubated for 48 hours with either 5 μM AG1295 or 5 μM Ara C. They were then assayed for annexin V binding as described. Cell populations from frozen samples have a fraction of blasts that die from the freeze-thaw cycle. Results are displayed as the percent of cells induced by drug to bind annexin V after first subtracting the baseline necrotic population from both the treatment and control (DMSO) groups.$

Apoptosis assay.

Blasts from a FLT3/ITD sample and a non-ITD sample were incubated for 48 hours with either 5 μM AG1295 or 5 μM Ara C. They were then assayed for annexin V binding as described. Cell populations from frozen samples have a fraction of blasts that die from the freeze-thaw cycle. Results are displayed as the percent of cells induced by drug to bind annexin V after first subtracting the baseline necrotic population from both the treatment and control (DMSO) groups.

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