Saturation of DFO or 311 with iron prevents their effects at reducing the protein levels of cyclin A, B1, D1, D2, and D3 and increasing cyclin E protein levels.

The SK-N-MC neuroepithelioma cell line was incubated with DFO (150 μM), the DFO-Fe complex (150 μM), 311 (25 μM), or the 311-Fe complex (25 μM) for 30 hours at 37°C. Western blot analysis was then performed as described in “Materials and methods.” Results shown are representative of 3 separate experiments performed.