Effect of chelator concentration, chelator incubation time, and complexation with Fe on the phosphorylation status of the retinoblastoma susceptibility gene product (pRb).

(A) Chelator concentration. The SK-N-MC neuroepithelioma cell line was incubated for 30 h at 37°C with DFO (5-150 μM) or 311 (1-25 μM). (B) Chelator incubation time. Cells were incubated for 2-30 h with DFO (150 μM) or 311 (25 μM). (C) Complexation with Fe. Cells were incubated with DFO (150 μM) or 311 (25 μM) or their preformed Fe complexes for 30 hours at 37°C. Western blot analysis was then performed after each of these incubation conditions, as described in “Materials and methods.” Results shown are representative of 3 separate experiments performed.