Fig. 1.
Fig. 1. GILZ inhibits anti-CD3–induced IL-2R expression, IL-2 production, and apoptosis. / (A) IL-2R expression in untreated (░) and anti-CD3–treated clones (▪) for 18 hours on plates coated with anti-CD3 (1 μg/mL), empty vector–transfected (PV5, PV6, PVT1, PVT2) clones, and GILZ-transfected (SG11, ST7, SG5, ST5, GIRL19) clones as evaluated by fluorescent anti–IL-2 mAb and flow cytometry analysis (see “Material and methods”). (B) IL-2 production (pg/mL) in untreated (░) and anti-CD3–treated (▪), empty vector–transfected (PV5, PV6, PVT1, PVT2), and GILZ-transfected (SG11, ST7, SG5, ST5, GIRL19) clones as evaluated by ELISA assay. (C) Percentage apoptosis in untreated (░) and anti-CD3–treated (▪), empty vector–transfected (PV5, PV6, PVT1, PVT2), and GILZ-transfected (SG11, ST7, SG5, ST5, GIRL19) clones as evaluated by PI assay.

GILZ inhibits anti-CD3–induced IL-2R expression, IL-2 production, and apoptosis.

(A) IL-2R expression in untreated (░) and anti-CD3–treated clones (▪) for 18 hours on plates coated with anti-CD3 (1 μg/mL), empty vector–transfected (PV5, PV6, PVT1, PVT2) clones, and GILZ-transfected (SG11, ST7, SG5, ST5, GIRL19) clones as evaluated by fluorescent anti–IL-2 mAb and flow cytometry analysis (see “Material and methods”). (B) IL-2 production (pg/mL) in untreated (░) and anti-CD3–treated (▪), empty vector–transfected (PV5, PV6, PVT1, PVT2), and GILZ-transfected (SG11, ST7, SG5, ST5, GIRL19) clones as evaluated by ELISA assay. (C) Percentage apoptosis in untreated (░) and anti-CD3–treated (▪), empty vector–transfected (PV5, PV6, PVT1, PVT2), and GILZ-transfected (SG11, ST7, SG5, ST5, GIRL19) clones as evaluated by PI assay.

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