GILZ inhibits p65/p52-dependent transactivation of a IgG/HIV-κB (pBIIXLUC) site-driven luciferase reporter.

Analysis of transcriptional activity was performed on transiently transfected 293 (A-C, G) or NTera-2 (D-F) cells. The indicated amounts of PMT2T-GILZ (0.1 pmol = 370 ng), PMT2T-p65 (0.3 pmol = 1.4 μg), and PMT2T-p52 (0.01 pmol = 47 ng), alone or in combination, were cotransfected with 15 μg (4 pmol) of the target plasmid pBIIXLUC. The values are expressed as fold increases of luciferase activity. Each transfection was performed in triplicate, and SD bars are shown. EC indicates endogenous control.