GILZ coimmunoprecipitates with p65 and p52.

NTera-2 cells were transfected with 6 μg of expression plasmids for GILZ, p65, and p52 either alone or in combination. Following transfection, the cells were metabolically labeled and whole-cell extracts used for immunoprecipitations with anti-GILZ, anti-p65, and anti-p52 antibodies, as indicated. Anti-GILZ immunoprecipitations were blocked with 400 ng of the GILZ fusion protein used to generate the antibody. The arrows indicate the immunoprecipitated proteins.