Fig. 10.
Fig. 10. TCR/CD3 triggering inhibits GILZ expression. / Northern (A,C) and Western (B) blot analysis of GILZ. (A,B) Lane 1: untreated thymocytes; lane 2: thymocytes treated for 6 hours on plates coated with anti-CD3 (1 μg/mL); lane 3: thymocytes treated with 100 nM DEX for 6 hours; lane 4: thymocytes treated for 6 hours with anti-CD3 plus DEX. For the Northern blot analysis, the filter hybridized with GILZ cDNA was exposed for autoradiography for 36 hours. (C) Lane 1: untreated thymocytes; lane 2: thymocytes treated for 6 hours on plates coated with anti-CD3 (1 μg/mL); lane 3: thymocytes treated with cyclosporin A (1 μg/mL) for 6 hours; lane 4: thymocytes treated for 6 hours with anti-CD3 plus cyclosporin A. Filter hybridized with GILZ cDNA was exposed for autoradiography for 3 days.

TCR/CD3 triggering inhibits GILZ expression.

Northern (A,C) and Western (B) blot analysis of GILZ. (A,B) Lane 1: untreated thymocytes; lane 2: thymocytes treated for 6 hours on plates coated with anti-CD3 (1 μg/mL); lane 3: thymocytes treated with 100 nM DEX for 6 hours; lane 4: thymocytes treated for 6 hours with anti-CD3 plus DEX. For the Northern blot analysis, the filter hybridized with GILZ cDNA was exposed for autoradiography for 36 hours. (C) Lane 1: untreated thymocytes; lane 2: thymocytes treated for 6 hours on plates coated with anti-CD3 (1 μg/mL); lane 3: thymocytes treated with cyclosporin A (1 μg/mL) for 6 hours; lane 4: thymocytes treated for 6 hours with anti-CD3 plus cyclosporin A. Filter hybridized with GILZ cDNA was exposed for autoradiography for 3 days.

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