Translocation of normal and WAS-immature DCs.

Translocation of WAS immature DCs on a fibronectin substratum is severely compromised. Scale bars represent 10 μm. Frames were taken at 60-minute intervals for up to 300 minutes. (A) Over time, normal immature DCs become polarized and begin random translocation over the substratum. In general, migrating cells have a well-developed broad and stable leading edge (arrows) with a fine uropod (arrowheads) that retracts into the cell body at sporadic intervals, resulting in net cell translocation. (B) In contrast, many WAS DCs elongate to an extreme degree. Persistent broad, leading-edge lamellipodia do not form, although small, transient extensions are formed randomly around the cell periphery (arrows). Cells fail to select a leading edge or a uropod, and net cell translocation over the substratum is severely compromised.