Normal immature DCs expressing a GFP-WASp fusion protein.

GFP-WASp fusion protein localizes to podosomes in normal immature DCs. Confocal images are shown in horizontal (panels A, B, E, F) and vertical (panels C, D, G, H) planes. TRITC-phalloidin staining of microinjected normal DCs is shown in left-hand panels (panels A, C, E, G). Right-hand panels show expression of EGFP (panels B, D) or EGFP-WASp (panels F, H). While EGFP shows a diffuse distribution within the cytoplasm (panels B, D), EGFP-WASp fusion protein in normal DCs is concentrated at sites of podosomal assembly (panels F, H).