β3-null (β3^−/−) mice are protected from FeCl₃-induced thrombosis of the carotid artery.

To determine the extent of temperature change during the cessation of blood flow, the arteries were occluded for 30 seconds with a vascular clamp before the application of FeCl₃ (arrow). Time to maximal temperature reduction occurred 5 minutes and 12 minutes after the initiation of injury in the β3^+/+ mouse and the β3^+/− mouse, respectively. Up to 30 minutes after the addition of FeCl₃, no temperature reduction occurred in the β3^−/− mouse, at which time the clamp was re-applied to document that the vessel was still patent.