mAb 350 recognizes the β-chain of mouse fibrin but does not react with fibrinogen.

Mouse fibrinogen (lane 1), mouse fibrin prepared in the absence of calcium to limit endogenous factor XIII–mediated cross-linking (lane 2), or mouse fibrin prepared in the presence of calcium to promote endogenous factor XIII–mediated cross-linking (lane 3) was separated by polyacrylamide gel electrophoresis and visualized with Coomassie staining (left) or subjected to immunoblot analysis with mAb 350 (right). γγ, γ-dimer; αα, α polymer.