Fig. 3.
Detection of cell division of the thymus-seeding cells after IV transfer of CFSE-labeled BM cells.
CFSE fluorescence intensity of the thymus-seeding cells (Ly-5.2+) was measured at the time indicated after BM transfer. Note that because BM represented a heterogeneous population of cells, the fluorescence intensity of initial labeling obtained was broader than with lymphocytes, and the division produced a wide spread of fluorescence rather than discrete peaks.