Reconstitution of NK and NKT cells in the spleen and lymph nodes of secondary recipients.

Thymus cells from the primary recipients 5 and 6 days after initial BM transfer were injected intravenously into secondary recipients and were then analyzed 4 weeks later. The procedures for cell transfer are the same as described in Figure. 5. Donor-derived NK and NKT cells were revealed by staining cells for Ly-5.2, together with CD3 and NK1.1. Expression of NK1.1 and CD3 on the donor BM-derived cells, gated as Ly-5.2⁺ cells, is shown.