Hematopoietic lineage reconstitution in the secondary Ly-5.1 recipients when the primary recipients received direct intrathymic injection of BM cells.

Thymus cells from primary Ly-5.1 recipients were harvested 3 days after direct intrathymic injection of T-cell– and mature B-cell–depleted BM cells from C57BL/6 (Ly-5.2) mice, then were transferred intravenously to secondary Ly-5.1 recipients. Cells from the thymus, spleen plus lymph nodes, and BM of the secondary recipients were analyzed 4 weeks after the secondary transfer, as in Figure 5. The result shown in this figure is a representative of 2 such experiments that gave similar results. Each experiment included 2 to 3 recipient mice.