FISH analysis of NUP98 rearrangement and chromosomal mapping of humanNSD1 gene.

(A) FISH using PAC 1173K1 (containing NUP98 gene) shows that this probe hybridizes to the normal chromosome 11, der(11) (arrow) and the der(5) (arrowhead), seen as red fluorescent signals in bone marrow metaphase from patient no. 3. The probe showing green fluorescent signals is the control 5p probe (cos113-1) used to identify both chromosomes 5. The same FISH result was also seen in metaphases from patient no. 1. (B) FISH using a cDNA clone of human NSD1 (Image clone 2621298) as a probe on normal peripheral blood metaphase shows that this novel gene localizes to 5q35 (red fluorescent signals) (arrowhead).