NUP98-NSD1 and NSD1-NUP98 fusion cDNA.

Nucleotide and amino acid sequences around the junction of (A)NUP98-NSD1 fusion transcript and (B) NSD1-NUP98fusion transcript. The NUP98 and NSD1 fusion points are shown by a vertical arrow. (C) RT-PCR assay forNUP98-NSD1 fusion mRNA. Primers NUP98-5 and NSD1-1 were used to amplify the 135 bp fusion mRNA. (D) RT-PCR assay forNSD1-NUP98 fusion mRNA. Primers NSD1-2 and NUP98-6 were used to amplify the 200 bp fusion mRNA. No specific products of the expected size are seen in the normal controls (C1-C2) and negative controls (H₂O) in panels C and D. MWM indicates 1 kb ladder molecular weight marker. (E) Schematic representations of the wild-type NUP98, NSD1, and fusion NUP98-NSD1, NSD1-NUP98 proteins. Functional domains indicated for NUP98 are FG repeat regions at the N-terminus and RNA binding domain at the C-terminus. Domains for NSD1 are N-terminus, nuclear receptor interaction domain (NID), PHD_I-IV fingers, SET and SAC domains, PHD_V finger, and cysteine-histidine (C/H)-rich motif, and C-terminus. Functional domains of NSD1 are similar to the murine Nsd1 as designated in reference 17. NUP98 and NSD1 fusion points are shown by a vertical arrow.