Doxorubicin up-regulates DR5 expression of MM-1S cells.

Immunoblotting analysis (A) of Dox-treated (250 or 500 ng/mL) MM-1S cells reveals dose-dependent up-regulation of DR5 expression, confirmed by flow cytometry (B). Dox also induces a small increase in DcR1 and decrease in DcR2 expression, by immunoblotting analysis (A).