Transient calcium mobilization and chemotaxis of MOLT4 cells in response to TECK.

(A) MOLT4 cells were loaded with Fura-2AM and stimulated with 100 nM TECK. Calcium flux was monitored by measuring relative fluorescence of Fura-2AM (i). Fura-2AM–loaded MOLT4 cells were stimulated with the indicated concentrations of TECK (1 to 100 nM), and fluorescence was monitored. The peak amplitude of the calcium response was plotted (ii). Representative data from 3 different experiments are shown. (B) Chemotactic responsiveness of MOLT4 cells to TECK. A half-million MOLT4 cells were placed into a transwell chamber and allowed to migrate in the presence of varying concentrations of TECK for 4 hours. The number of cells were counted by a hemocytometer. MOLT4 cells were also pretreated with pertussis toxin (PT) (1 μg/mL) for 1 hour and used for chemotaxis in response to TECK. SD was calculated from 3 independent experiments.