Effect of the CCR9/TECK or CXCR4/SDF-1 interaction on CHX-induced apoptosis as well as on Fas-mediated apoptosis.

MOLT4 cells were serum starved overnight and treated with CHX (5 μM) or CH-11 (500 ng/mL), alone or in combination, in the presence or absence of TECK (1 μg/mL), for 6 hours. As a control, Z-VAD-FMK, a caspase inhibitor, was used for blocking apoptosis. Apoptotic cells were measured by annexin-V staining by means of flow cytometry. The percentage listed under each flow analysis is the percentage of apoptotic cells, as seen in the lower right-hand quadrant. Shown are representative data from 3 different experiments.