TECK and SDF-1 suppress CHX- and CH-11–induced caspase-3 activation.

Cells were treated as described in Figure 5. They were then permeabilized and fixed as indicated in “Materials and methods” and subsequently stained for intracellular activated (cleaved) caspase 3. Activated caspase-3–specific fluorescence intensity was measured by flow cytometry. The percentage of cells with activated caspase 3 was quantitated from relative-frequency histograms (panels A and B) and is shown in panels C and D. Panels A and B are representative of 2 separate experiments, whereas panels C and D are an average of the 2 separate experiments.