Fig. 2.
CD40 cross-linking of tumor cells induces the secretion of the chemokines MDC and TARC.
Leukemia cells were cultured in control medium (open circles) or sCD40L (solid triangles) for 3 days. Supernatants were collected from nonpurified leukemia cells cultured at 107 cells/mL (or more) or at 3 to 5 × 106 cells/mL, or from leukemia cells purified by sorting and cultured at 3 × 106cells/mL. The dotted lines indicate the limit of detection (LD) of the ELISA, which was 0.25 ng/mL for MDC and 0.13 ng/mL for TARC. For the purpose of clarity, the supernatants in which MDC and TARC were below the detection limit are represented as below the 0 level.
